Breaking Down Barriers: What Happens When the Vaginal Microbiome Attacks

“Even when bacterial vaginosis is identified and treated with antibiotics, recurrences occur in most individuals within a year,” said Lewis.

To understand how these bacteria impact vaginal health, the researchers studied the epithelial cells that line the vagina. Because the surface of epithelial cells comes into contact with bacteria and other microbes, it is densely coated with sugar chains, called glycans. Glycans play key roles in cell biology and disease, such as protecting against microbial invasion and helping cells adhere to each other. However, glycans can also be a food source for bacteria.

“We knew that bacterial species implicated in bacterial vaginosis can feed on glycans in secreted mucus. The current study allowed us to look directly at what those bacteria are then doing to the vaginal epithelial surface landscape on a biochemical and microscopic level,” said co-corresponding author Amanda Lewis, PhD, professor in the Department of Obstetrics, Gynecology & Reproductive Sciences at UC San Diego School of Medicine.

The researchers obtained epithelial cells derived from human vaginal specimens and used them to explore glycan dynamics. With a combination of biochemistry and microscopy techniques, they discovered that in bacterial vaginosis, bacteria release enzymes called sialidases that partially dismantle protective glycan molecules on the surface of epithelial cells. The researchers were also able to induce a bacterial-vaginosis-like state in ‘normal’ epithelial cells by treating them directly with sialidase enzymes produced in the laboratory.

“The fact that we were able to replicate some of the effects of bacterial vaginosis suggests that we may be on the right track to finding a common cellular origin for the various complications associated with this condition,” said Amanda Lewis.

Studying the surface of vaginal epithelial cells at this level of biochemical detail could help make diagnosing bacterial vaginosis easier. The authors suggest differences in glycosylation patterns could also help identify subsets of people with the condition who may be at the greatest risk for negative health outcomes, including recurrence.

“We now have a blueprint of the glycans present on epithelial cells in the vagina, and we showed that these glycans are shaped by the bacteria that live there,” said Warren Lewis. “However, it will take more work to fully understand the functions of glycans in the vaginal epithelium and how bacterial vaginosis impacts those functions.”

As research on the mechanisms of bacterial vaginosis continue, clinicians urge people with vaginas to familiarize themselves with the symptoms of bacterial vaginosis and avoid douching or using scented products, which could result in further microbial imbalances.

Co-authors include: Kavita Agarwal, Biswa Choudhury, Sydney R. Morrill, Daisy Chilin-Fuentes, Sara B. Rosenthal, Kathleen M. Fisch at UC San Diego, Lloyd S. Robinson at University of Washington School of Medicine, Yasmine Bouchibiti and Carlito B. Lebrilla at University of California Davis, Jenifer E. Allsworth at the University of Missouri, and  Jeffrey F. Peipert at Indiana University School of Medicine.

This study was funded, in part, by the National Institutes of Health (grants R01 AI114635, R01 AI127554 and UL1TR001442), the Burrroughs Wellcome Fund Preterm Birth Initiative and the University of California through the UC Glycosciences Consortium for Women’s Health.