Borderzone Breakthrough: A New Source of Cardiac Inflammation

Inflammation after MI is classically credited to professional immune cells like neutrophils and macrophages that infiltrate the infarcted heart and respond to molecules in the debris of dying cells. So the team was surprised when they discovered that the proinflammatory “type I interferon (IFN) response” was activated, not in the infarct where immune cells were concentrated, but instead in the borderzone, surrounding the infarct.

The borderzone has been a fascinating yet understudied area of the infarcted heart. It is where surviving heart muscle cells attempt to stabilize and even proliferate after being disconnected from their dying neighbor cells. Unfortunately, the borderzone has proven a challenging region to study because it is not easily isolated from the rest of the heart. Researchers overcame this obstacle using methods they recently reported based on single cell RNAseq and spatial transcriptomics where cells of the borderzone are recognized based on their patterns of gene expression (https://www.nature.com/articles/s44161-022-00160-3).

To determine which cell type initiates borderzone inflammation, the team created a library of conditional knockout mice, each unable to initiate IFN signaling in a different cell type. To their surprise, heart muscle cells called cardiomyocytes emerged as the dominant initiators of borderzone IFN signaling. They found that mechanically stressed cardiomyocytes in the borderzone frequently suffered nuclear envelope rupture, which allowed escape of nuclear DNA and sensing by cytosolic DNA sensors, leading to activation of IFN signaling. This in turn caused mechanical weakening of the heart wall and made it vulnerable to dilation, thinning, and rupture, providing a mechanistic explanation for the team’s previous reported observation that mice lacking IFN responses exhibited improved survival after MI (https://www.nature.com/articles/nm.4428).

“In the hospital, we care for patients with heart attacks and heart failure every day. New therapeutic targets for MI with the potential to prevent development of heart failure are incredibly important, said Dr. King, senior author of the study an on the faculty in the Shu Chien Gene Lay Department of Bioengineering and the Division of Cardiology at UC San Diego.

Many questions remain, however the newly reported findings suggest that limiting mechanical stress at the borderzone, inhibiting DNA sensing, and preventing type I IFN signaling may represent new opportunities for patients to avoid development of heart failure after MI.

Funding support for the study came, in part, from the NIH DP2 New Innovator Award.

Nature paper: Spatially clustered type I interferon responses at injury borderzones